Construct: shRNA TRCN0000294593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053110.4-2364s21c1
DNA Barcode:
GTGTACATATTCTACTCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpnmb (93695)
Gene Description:
glycoprotein (transmembrane) nmb
Transcript:
RefSeq NM_053110.4 (CURRENT)
Match location:
Position 2364 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTACATATTCTACTCATTA
Hairpin Sequence:
5'-CCGG-GTGTACATATTCTACTCATTA-CTCGAG-TAATGAGTAGAATATGTACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTACATATTCTACTCATTACTCGAGTAATGAGTAGAATATGTACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTACATATTCTACTCATTACTCGAGTAATGAGTAGAATATGTACAC-3'

Other clones with same target sequence:

(none)