Construct: shRNA TRCN0000294685

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010028.3-187s21c1
DNA Barcode:
AGCAAGCAAAGGGCGTTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ddx3x (13205)
Gene Description:
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked
Transcript:
RefSeq NM_010028.3 (CURRENT)
Match location:
Position 187 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294685)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAAGCAAAGGGCGTTATAT
Hairpin Sequence:
5'-CCGG-AGCAAGCAAAGGGCGTTATAT-CTCGAG-ATATAACGCCCTTTGCTTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAAGCAAAGGGCGTTATATCTCGAGATATAACGCCCTTTGCTTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAAGCAAAGGGCGTTATATCTCGAGATATAACGCCCTTTGCTTGCT-3'

Other clones with same target sequence:

(none)