Construct: shRNA TRCN0000294819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009703.2-1210s21c1
DNA Barcode:
AGACACGGCATGTCAACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Araf (11836)
Gene Description:
Araf proto-oncogene, serine/threonine kinase
Transcript:
RefSeq NM_009703.2 (CURRENT)
Match location:
Position 1210 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACACGGCATGTCAACATTT
Hairpin Sequence:
5'-CCGG-AGACACGGCATGTCAACATTT-CTCGAG-AAATGTTGACATGCCGTGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACACGGCATGTCAACATTTCTCGAGAAATGTTGACATGCCGTGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACACGGCATGTCAACATTTCTCGAGAAATGTTGACATGCCGTGTCT-3'

Other clones with same target sequence:

(none)