Construct: shRNA TRCN0000294948

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011941.3-4086s21c1
DNA Barcode:
GCCAAGCTAGCGCGTAGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapkbp1 (26390)
Gene Description:
mitogen-activated protein kinase binding protein 1
Transcript:
RefSeq NM_011941.3 (CURRENT)
Match location:
Position 4086 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000294948)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGCTAGCGCGTAGTATT
Hairpin Sequence:
5'-CCGG-GCCAAGCTAGCGCGTAGTATT-CTCGAG-AATACTACGCGCTAGCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGCTAGCGCGTAGTATTCTCGAGAATACTACGCGCTAGCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGCTAGCGCGTAGTATTCTCGAGAATACTACGCGCTAGCTTGGC-3'

Other clones with same target sequence:

(none)