Construct: shRNA TRCN0000295026

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010015.4-396s21c1
DNA Barcode:
CCTGCACCTTGTCGTCATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dad1 (13135)
Gene Description:
defender against cell death 1
Transcript:
RefSeq NM_010015.4 (CURRENT)
Match location:
Position 396 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295026)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCACCTTGTCGTCATGAA
Hairpin Sequence:
5'-CCGG-CCTGCACCTTGTCGTCATGAA-CTCGAG-TTCATGACGACAAGGTGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCACCTTGTCGTCATGAACTCGAGTTCATGACGACAAGGTGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCACCTTGTCGTCATGAACTCGAGTTCATGACGACAAGGTGCAGG-3'

Other clones with same target sequence:

(none)