Construct: shRNA TRCN0000295307

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025898.3-150s21c1
DNA Barcode:
AGGAAGCATGCGAGATCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Napa (108124)
Gene Description:
N-ethylmaleimide sensitive fusion protein attachment protein alpha
Transcript:
RefSeq NM_025898.3 (CURRENT)
Match location:
Position 150 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295307)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAAGCATGCGAGATCTATG
Hairpin Sequence:
5'-CCGG-AGGAAGCATGCGAGATCTATG-CTCGAG-CATAGATCTCGCATGCTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAAGCATGCGAGATCTATGCTCGAGCATAGATCTCGCATGCTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAAGCATGCGAGATCTATGCTCGAGCATAGATCTCGCATGCTTCCT-3'

Other clones with same target sequence:

(none)