Construct: shRNA TRCN0000295444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133969.2-2229s21c1
DNA Barcode:
GTCATCAGAGATATCAGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyp4v3 (102294)
Gene Description:
cytochrome P450, family 4, subfamily v, polypeptide 3
Transcript:
RefSeq NM_133969.2 (CURRENT)
Match location:
Position 2229 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATCAGAGATATCAGTTAT
Hairpin Sequence:
5'-CCGG-GTCATCAGAGATATCAGTTAT-CTCGAG-ATAACTGATATCTCTGATGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATCAGAGATATCAGTTATCTCGAGATAACTGATATCTCTGATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATCAGAGATATCAGTTATCTCGAGATAACTGATATCTCTGATGAC-3'

Other clones with same target sequence:

(none)