Construct: shRNA TRCN0000295891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000321.2-1516s21c1
DNA Barcode:
TCGCTTGTATTACCGAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RB1 (5925)
Gene Description:
RB transcriptional corepressor 1
Transcript:
RefSeq NM_000321.2 (CURRENT)
Match location:
Position 1516 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000295891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGCTTGTATTACCGAGTAAT
Hairpin Sequence:
5'-CCGG-TCGCTTGTATTACCGAGTAAT-CTCGAG-ATTACTCGGTAATACAAGCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGCTTGTATTACCGAGTAATCTCGAGATTACTCGGTAATACAAGCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGCTTGTATTACCGAGTAATCTCGAGATTACTCGGTAATACAAGCGA-3'

Other clones with same target sequence:

(none)