Construct: shRNA TRCN0000296694

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005386.2-1723s21c1
DNA Barcode:
TGCTGCTAGTCGTAGTCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACTR2 (10097)
Gene Description:
actin related protein 2
Transcript:
RefSeq NM_001005386.2 (CURRENT)
Match location:
Position 1723 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000296694)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTGCTAGTCGTAGTCTTTA
Hairpin Sequence:
5'-CCGG-TGCTGCTAGTCGTAGTCTTTA-CTCGAG-TAAAGACTACGACTAGCAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTGCTAGTCGTAGTCTTTACTCGAGTAAAGACTACGACTAGCAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTGCTAGTCGTAGTCTTTACTCGAGTAAAGACTACGACTAGCAGCA-3'

Other clones with same target sequence:

(none)