Construct: shRNA TRCN0000297223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026718.2-687s21c1
DNA Barcode:
GAACACTTCGATCTTTCCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankrd13a (68420)
Gene Description:
ankyrin repeat domain 13a
Transcript:
RefSeq NM_026718.2 (CURRENT)
Match location:
Position 687 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACACTTCGATCTTTCCCAA
Hairpin Sequence:
5'-CCGG-GAACACTTCGATCTTTCCCAA-CTCGAG-TTGGGAAAGATCGAAGTGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACACTTCGATCTTTCCCAACTCGAGTTGGGAAAGATCGAAGTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACACTTCGATCTTTCCCAACTCGAGTTGGGAAAGATCGAAGTGTTC-3'

Other clones with same target sequence:

  1. TRCN0000178073