Construct: shRNA TRCN0000297311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001130965.1-1965s21c1
DNA Barcode:
GCTGTTCTGAAACTTACGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUN1 (23353)
Gene Description:
Sad1 and UNC84 domain containing 1
Transcript:
RefSeq NM_001130965.1 (NON-CURRENT)
Match location:
Position 1965 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTCTGAAACTTACGAAA
Hairpin Sequence:
5'-CCGG-GCTGTTCTGAAACTTACGAAA-CTCGAG-TTTCGTAAGTTTCAGAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTCTGAAACTTACGAAACTCGAGTTTCGTAAGTTTCAGAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTCTGAAACTTACGAAACTCGAGTTTCGTAAGTTTCAGAACAGC-3'

Other clones with same target sequence:

  1. TRCN0000133655