Construct: shRNA TRCN0000133655

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_379766.1-2453s1c1
DNA Barcode:
GCTGTTCTGAAACTTACGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUN1 (23353)
Gene Description:
Sad1 and UNC84 domain containing 1
Transcript:
RefSeq XM_379766.1 (NON-CURRENT)
Match location:
Position 2453 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000133655)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTCTGAAACTTACGAAA
Hairpin Sequence:
5'-CCGG-GCTGTTCTGAAACTTACGAAA-CTCGAG-TTTCGTAAGTTTCAGAACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTCTGAAACTTACGAAACTCGAGTTTCGTAAGTTTCAGAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTCTGAAACTTACGAAACTCGAGTTTCGTAAGTTTCAGAACAGC-3'

Other clones with same target sequence:

  1. TRCN0000297311