Construct: shRNA TRCN0000297869

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028259.4-295s21c1
DNA Barcode:
GCATGGAACATTGTGAGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rps6kb1 (72508)
Gene Description:
ribosomal protein S6 kinase, polypeptide 1
Transcript:
RefSeq NM_028259.4 (CURRENT)
Match location:
Position 295 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000297869)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGGAACATTGTGAGAAAT
Hairpin Sequence:
5'-CCGG-GCATGGAACATTGTGAGAAAT-CTCGAG-ATTTCTCACAATGTTCCATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGGAACATTGTGAGAAATCTCGAGATTTCTCACAATGTTCCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGGAACATTGTGAGAAATCTCGAGATTTCTCACAATGTTCCATGC-3'

Other clones with same target sequence:

  1. TRCN0000022904