Construct: shRNA TRCN0000298086

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026479.4-281s21c1
DNA Barcode:
GTACCTCTAGCAGTGACAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zcchc10 (67966)
Gene Description:
zinc finger, CCHC domain containing 10
Transcript:
RefSeq NM_026479.4 (CURRENT)
Match location:
Position 281 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298086)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACCTCTAGCAGTGACAGTT
Hairpin Sequence:
5'-CCGG-GTACCTCTAGCAGTGACAGTT-CTCGAG-AACTGTCACTGCTAGAGGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACCTCTAGCAGTGACAGTTCTCGAGAACTGTCACTGCTAGAGGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACCTCTAGCAGTGACAGTTCTCGAGAACTGTCACTGCTAGAGGTAC-3'

Other clones with same target sequence:

  1. TRCN0000182550