Construct: shRNA TRCN0000298304

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019927.2-1113s21c1
DNA Barcode:
CCAGAACTGTAAAGCAGAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Arih1 (23806)
Gene Description:
ariadne RBR E3 ubiquitin protein ligase 1
Transcript:
RefSeq NM_019927.2 (CURRENT)
Match location:
Position 1113 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298304)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAACTGTAAAGCAGAATT
Hairpin Sequence:
5'-CCGG-CCAGAACTGTAAAGCAGAATT-CTCGAG-AATTCTGCTTTACAGTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAACTGTAAAGCAGAATTCTCGAGAATTCTGCTTTACAGTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAACTGTAAAGCAGAATTCTCGAGAATTCTGCTTTACAGTTCTGG-3'

Other clones with same target sequence:

  1. TRCN0000041012