Construct: shRNA TRCN0000298381

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029397.3-1103s21c1
DNA Barcode:
CCTGTGTTTCTGGGTCCATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm12 (75710)
Gene Description:
RNA binding motif protein 12
Transcript:
RefSeq NM_029397.3 (CURRENT)
Match location:
Position 1103 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000298381)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGTTTCTGGGTCCATTA
Hairpin Sequence:
5'-CCGG-CCTGTGTTTCTGGGTCCATTA-CTCGAG-TAATGGACCCAGAAACACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGTTTCTGGGTCCATTACTCGAGTAATGGACCCAGAAACACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGTTTCTGGGTCCATTACTCGAGTAATGGACCCAGAAACACAGG-3'

Other clones with same target sequence:

  1. TRCN0000102486