Construct: shRNA TRCN0000300353

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001848.2-4055s21c1
DNA Barcode:
GCTGTGTCTTACTAGAAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL6A1 (1291)
Gene Description:
collagen type VI alpha 1 chain
Transcript:
RefSeq NM_001848.2 (NON-CURRENT)
Match location:
Position 4055 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000300353)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGTCTTACTAGAAACAA
Hairpin Sequence:
5'-CCGG-GCTGTGTCTTACTAGAAACAA-CTCGAG-TTGTTTCTAGTAAGACACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGTCTTACTAGAAACAACTCGAGTTGTTTCTAGTAAGACACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGTCTTACTAGAAACAACTCGAGTTGTTTCTAGTAAGACACAGC-3'

Other clones with same target sequence:

  1. TRCN0000116957