Construct: shRNA TRCN0000301306
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_008838.1-786s21c1
- DNA Barcode:
- GAGGAGAAAGGAGAGAAGTTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Pigf (18701)
- Gene Description:
- phosphatidylinositol glycan anchor biosynthesis, class F
- Transcript:
- RefSeq NM_008838.1 (CURRENT)
- Match location:
- Position 786 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000301306)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAGGAGAAAGGAGAGAAGTTA
- Hairpin Sequence:
-
5'-CCGG-GAGGAGAAAGGAGAGAAGTTA-CTCGAG-TAACTTCTCTCCTTTCTCCTC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAGGAGAAAGGAGAGAAGTTACTCGAGTAACTTCTCTCCTTTCTCCTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAGGAGAAAGGAGAGAAGTTACTCGAGTAACTTCTCTCCTTTCTCCTC-3'
Other clones with same target sequence:
-
TRCN0000077128
