Transcript: Mouse NM_008838.1

Mus musculus phosphatidylinositol glycan anchor biosynthesis, class F (Pigf), mRNA.

Source:
NCBI, updated 2017-06-25
Taxon:
Mus musculus (mouse)
Gene:
Pigf (18701)
Length:
980
CDS:
126..785

Additional Resources:

NCBI RefSeq record:
NM_008838.1
NBCI Gene record:
Pigf (18701)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008838.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000077131 GTCAATCTCTTGTCGTACTTA pLKO.1 285 CDS 100% 5.625 7.875 N Pigf n/a
2 TRCN0000301305 GTCAATCTCTTGTCGTACTTA pLKO_005 285 CDS 100% 5.625 7.875 N Pigf n/a
3 TRCN0000077132 GTACCTTGTCTCTGTTTGTTA pLKO.1 498 CDS 100% 0.000 0.000 N Pigf n/a
4 TRCN0000301303 GTACCTTGTCTCTGTTTGTTA pLKO_005 498 CDS 100% 0.000 0.000 N Pigf n/a
5 TRCN0000077129 CGTGGACAACTTCTCAGTATT pLKO.1 215 CDS 100% 13.200 9.240 N Pigf n/a
6 TRCN0000331719 CGTGGACAACTTCTCAGTATT pLKO_005 215 CDS 100% 13.200 9.240 N Pigf n/a
7 TRCN0000077130 CCTATTCCACTGGATTGGGAA pLKO.1 639 CDS 100% 2.640 1.848 N Pigf n/a
8 TRCN0000077128 GAGGAGAAAGGAGAGAAGTTA pLKO.1 786 CDS 100% 5.625 3.375 N Pigf n/a
9 TRCN0000301306 GAGGAGAAAGGAGAGAAGTTA pLKO_005 786 CDS 100% 5.625 3.375 N Pigf n/a
10 TRCN0000153818 CAAACCTCAAAGCATGGCTAA pLKO.1 523 CDS 100% 4.050 2.835 N PIGF n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008838.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01199 pDONR223 100% 86.2% 87.6% None (many diffs) n/a
2 ccsbBroad304_01199 pLX_304 0% 86.2% 87.6% V5 (many diffs) n/a
3 TRCN0000475035 TCGCCCCCATTTTCCGGGCAACCG pLX_317 12.7% 86.2% 87.6% V5 (many diffs) n/a
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