Construct: shRNA TRCN0000301364

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028810.2-295s21c1
DNA Barcode:
CAGAACGTGAAATGCAAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnd3 (74194)
Gene Description:
Rho family GTPase 3
Transcript:
RefSeq NM_028810.2 (CURRENT)
Match location:
Position 295 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301364)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAACGTGAAATGCAAGATA
Hairpin Sequence:
5'-CCGG-CAGAACGTGAAATGCAAGATA-CTCGAG-TATCTTGCATTTCACGTTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAACGTGAAATGCAAGATACTCGAGTATCTTGCATTTCACGTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAACGTGAAATGCAAGATACTCGAGTATCTTGCATTTCACGTTCTG-3'

Other clones with same target sequence:

  1. TRCN0000077332