Construct: shRNA TRCN0000301389

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007569.2-377s21c1
DNA Barcode:
CGGTGTCCTTCATCTCCAAGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Btg1 (12226)
Gene Description:
B cell translocation gene 1, anti-proliferative
Transcript:
RefSeq NM_007569.2 (CURRENT)
Match location:
Position 377 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301389)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGTCCTTCATCTCCAAGT
Hairpin Sequence:
5'-CCGG-CGGTGTCCTTCATCTCCAAGT-CTCGAG-ACTTGGAGATGAAGGACACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGTCCTTCATCTCCAAGTCTCGAGACTTGGAGATGAAGGACACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGTCCTTCATCTCCAAGTCTCGAGACTTGGAGATGAAGGACACCG-3'

Other clones with same target sequence:

  1. TRCN0000055300