Transcript: Mouse NM_007569.2

Mus musculus B cell translocation gene 1, anti-proliferative (Btg1), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Btg1 (12226)
Length:
5010
CDS:
325..840

Additional Resources:

NCBI RefSeq record:
NM_007569.2
NBCI Gene record:
Btg1 (12226)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_007569.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000304264 CAACGTGCAAATGGTAGACAG pLKO_005 738 CDS 100% 4.050 5.670 N Btg1 n/a
2 TRCN0000304262 CAGGTTAAGATATAGTCTATT pLKO_005 833 CDS 100% 13.200 9.240 N Btg1 n/a
3 TRCN0000304263 CCAGACCTTATACCCAATTTG pLKO_005 1138 3UTR 100% 13.200 9.240 N Btg1 n/a
4 TRCN0000273342 GGAGCTGCTGGCAGAACATTA pLKO_005 459 CDS 100% 13.200 9.240 N BTG1 n/a
5 TRCN0000273341 ATGATGACTGTATCAGGTTAA pLKO_005 820 CDS 100% 10.800 7.560 N BTG1 n/a
6 TRCN0000055301 CATAAGATGGATCCTCTGATT pLKO.1 544 CDS 100% 4.950 3.465 N Btg1 n/a
7 TRCN0000310861 TGAGCAGTCAGGAGTTGTTCA pLKO_005 590 CDS 100% 4.950 3.465 N Btg1 n/a
8 TRCN0000055302 CAGAACATTACAAACATCACT pLKO.1 470 CDS 100% 3.000 2.100 N Btg1 n/a
9 TRCN0000055298 CCGTTGTATTCGCATCAACCA pLKO.1 525 CDS 100% 2.640 1.848 N Btg1 n/a
10 TRCN0000055299 GCAAGGGATCAGGTTACCGTT pLKO.1 509 CDS 100% 2.640 1.848 N Btg1 n/a
11 TRCN0000055300 CGGTGTCCTTCATCTCCAAGT pLKO.1 377 CDS 100% 4.050 2.430 N Btg1 n/a
12 TRCN0000301389 CGGTGTCCTTCATCTCCAAGT pLKO_005 377 CDS 100% 4.050 2.430 N Btg1 n/a
13 TRCN0000087901 CGCATCAACCATAAGATGGAT pLKO.1 535 CDS 100% 3.000 1.500 Y Btg1-ps2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007569.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00179 pDONR223 100% 96.8% 100% None (many diffs) n/a
2 ccsbBroad304_00179 pLX_304 98.3% 96.8% 100% V5 (many diffs) n/a
3 TRCN0000475091 CTTGGACATTTAAAAGATAACAGT pLX_317 48.5% 96.6% 99.4% V5 (many diffs) n/a
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