Construct: shRNA TRCN0000301400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019743.3-236s21c1
DNA Barcode:
CCAGGAAACCTCGCATCAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rybp (56353)
Gene Description:
RING1 and YY1 binding protein
Transcript:
RefSeq NM_019743.3 (CURRENT)
Match location:
Position 236 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAAACCTCGCATCAATT
Hairpin Sequence:
5'-CCGG-CCAGGAAACCTCGCATCAATT-CTCGAG-AATTGATGCGAGGTTTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAAACCTCGCATCAATTCTCGAGAATTGATGCGAGGTTTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAAACCTCGCATCAATTCTCGAGAATTGATGCGAGGTTTCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000077219