Construct: shRNA TRCN0000301628

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009671.5-62s21c1
DNA Barcode:
GATGCTTCTAAGACAAGAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankfy1 (11736)
Gene Description:
ankyrin repeat and FYVE domain containing 1
Transcript:
RefSeq NM_009671.5 (CURRENT)
Match location:
Position 62 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301628)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCTTCTAAGACAAGAGTA
Hairpin Sequence:
5'-CCGG-GATGCTTCTAAGACAAGAGTA-CTCGAG-TACTCTTGTCTTAGAAGCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCTTCTAAGACAAGAGTACTCGAGTACTCTTGTCTTAGAAGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCTTCTAAGACAAGAGTACTCGAGTACTCTTGTCTTAGAAGCATC-3'

Other clones with same target sequence:

  1. TRCN0000071462