Construct: shRNA TRCN0000301630

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009671.5-212s21c1
DNA Barcode:
GCAAGAGCAGTACAGTGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankfy1 (11736)
Gene Description:
ankyrin repeat and FYVE domain containing 1
Transcript:
RefSeq NM_009671.5 (CURRENT)
Match location:
Position 212 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301630)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGAGCAGTACAGTGATTT
Hairpin Sequence:
5'-CCGG-GCAAGAGCAGTACAGTGATTT-CTCGAG-AAATCACTGTACTGCTCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGAGCAGTACAGTGATTTCTCGAGAAATCACTGTACTGCTCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGAGCAGTACAGTGATTTCTCGAGAAATCACTGTACTGCTCTTGC-3'

Other clones with same target sequence:

  1. TRCN0000071461