Construct: shRNA TRCN0000301886

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011416.2-4260s21c1
DNA Barcode:
GCTGAGAAGTTGTCACCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smarca2 (67155)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Transcript:
RefSeq NM_011416.2 (CURRENT)
Match location:
Position 4260 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301886)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAGAAGTTGTCACCAAAT
Hairpin Sequence:
5'-CCGG-GCTGAGAAGTTGTCACCAAAT-CTCGAG-ATTTGGTGACAACTTCTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAGAAGTTGTCACCAAATCTCGAGATTTGGTGACAACTTCTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAGAAGTTGTCACCAAATCTCGAGATTTGGTGACAACTTCTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000071401