Construct: shRNA TRCN0000301904

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013836.3-4800s21c1
DNA Barcode:
GCTGAAGAGAAAGAGAACGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tcf20 (21411)
Gene Description:
transcription factor 20
Transcript:
RefSeq NM_013836.3 (CURRENT)
Match location:
Position 4800 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301904)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAGAGAAAGAGAACGAT
Hairpin Sequence:
5'-CCGG-GCTGAAGAGAAAGAGAACGAT-CTCGAG-ATCGTTCTCTTTCTCTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAGAGAAAGAGAACGATCTCGAGATCGTTCTCTTTCTCTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAGAGAAAGAGAACGATCTCGAGATCGTTCTCTTTCTCTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000085167