Construct: shRNA TRCN0000301978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011416.2-4308s21c1
DNA Barcode:
GCCATCATTGATACTGTGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smarca2 (67155)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Transcript:
RefSeq NM_011416.2 (CURRENT)
Match location:
Position 4308 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000301978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCATTGATACTGTGATA
Hairpin Sequence:
5'-CCGG-GCCATCATTGATACTGTGATA-CTCGAG-TATCACAGTATCAATGATGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCATTGATACTGTGATACTCGAGTATCACAGTATCAATGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCATTGATACTGTGATACTCGAGTATCACAGTATCAATGATGGC-3'

Other clones with same target sequence:

  1. TRCN0000071399