Construct: shRNA TRCN0000302014

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146074.1-1147s21c1
DNA Barcode:
CGTGACTTTACTATCCAGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tfb1m (224481)
Gene Description:
transcription factor B1, mitochondrial
Transcript:
RefSeq NM_146074.1 (CURRENT)
Match location:
Position 1147 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302014)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGACTTTACTATCCAGCTT
Hairpin Sequence:
5'-CCGG-CGTGACTTTACTATCCAGCTT-CTCGAG-AAGCTGGATAGTAAAGTCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGACTTTACTATCCAGCTTCTCGAGAAGCTGGATAGTAAAGTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGACTTTACTATCCAGCTTCTCGAGAAGCTGGATAGTAAAGTCACG-3'

Other clones with same target sequence:

  1. TRCN0000081978