Transcript: Mouse NM_146074.1

Mus musculus transcription factor B1, mitochondrial (Tfb1m), mRNA.

Source:
NCBI, updated 2017-05-13
Taxon:
Mus musculus (mouse)
Gene:
Tfb1m (224481)
Length:
1360
CDS:
1..1038

Additional Resources:

NCBI RefSeq record:
NM_146074.1
NBCI Gene record:
Tfb1m (224481)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_146074.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000081978 CGTGACTTTACTATCCAGCTT pLKO.1 1147 3UTR 100% 2.640 3.696 N Tfb1m n/a
2 TRCN0000302014 CGTGACTTTACTATCCAGCTT pLKO_005 1147 3UTR 100% 2.640 3.696 N Tfb1m n/a
3 TRCN0000081981 CTGGCAGTCTAGCAGATGTTT pLKO.1 152 CDS 100% 5.625 3.938 N Tfb1m n/a
4 TRCN0000302015 CTGGCAGTCTAGCAGATGTTT pLKO_005 152 CDS 100% 5.625 3.938 N Tfb1m n/a
5 TRCN0000081979 CCTCTTTACAATCCCAGGAAA pLKO.1 624 CDS 100% 4.950 3.465 N Tfb1m n/a
6 TRCN0000302020 CCTCTTTACAATCCCAGGAAA pLKO_005 624 CDS 100% 4.950 3.465 N Tfb1m n/a
7 TRCN0000081982 ACTCGCTTTATCCCAGGGTTA pLKO.1 262 CDS 100% 4.050 2.835 N Tfb1m n/a
8 TRCN0000081980 GCCCTTTCGTTTATGGCAGAA pLKO.1 488 CDS 100% 4.050 2.835 N Tfb1m n/a
9 TRCN0000302078 GCCCTTTCGTTTATGGCAGAA pLKO_005 488 CDS 100% 4.050 2.835 N Tfb1m n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_146074.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03207 pDONR223 100% 85.2% 84.6% None (many diffs) n/a
2 ccsbBroad304_03207 pLX_304 0% 85.2% 84.6% V5 (many diffs) n/a
3 TRCN0000473484 ACTGCATTCTTTTGTGGAGTTTCA pLX_317 36% 85.2% 84.6% V5 (many diffs) n/a
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