Construct: shRNA TRCN0000302330

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030238.2-5192s21c1
DNA Barcode:
GCATCATCTTAAATGAGGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dync1h1 (13424)
Gene Description:
dynein cytoplasmic 1 heavy chain 1
Transcript:
RefSeq NM_030238.2 (CURRENT)
Match location:
Position 5192 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302330)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCATCTTAAATGAGGATA
Hairpin Sequence:
5'-CCGG-GCATCATCTTAAATGAGGATA-CTCGAG-TATCCTCATTTAAGATGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCATCTTAAATGAGGATACTCGAGTATCCTCATTTAAGATGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCATCTTAAATGAGGATACTCGAGTATCCTCATTTAAGATGATGC-3'

Other clones with same target sequence:

  1. TRCN0000091640