Construct: shRNA TRCN0000302338

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025609.2-2598s21c1
DNA Barcode:
GCCCTTCCATCAGGATTAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tab1 (66513)
Gene Description:
TGF-beta activated kinase 1/MAP3K7 binding protein 1
Transcript:
RefSeq NM_025609.2 (CURRENT)
Match location:
Position 2598 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302338)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTCCATCAGGATTAAAT
Hairpin Sequence:
5'-CCGG-GCCCTTCCATCAGGATTAAAT-CTCGAG-ATTTAATCCTGATGGAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTCCATCAGGATTAAATCTCGAGATTTAATCCTGATGGAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTCCATCAGGATTAAATCTCGAGATTTAATCCTGATGGAAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000088798