Construct: shRNA TRCN0000302500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176979.5-220s21c1
DNA Barcode:
GCTTTATATCTGTGACCGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Topbp1 (235559)
Gene Description:
topoisomerase (DNA) II binding protein 1
Transcript:
RefSeq NM_176979.5 (CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTATATCTGTGACCGTTT
Hairpin Sequence:
5'-CCGG-GCTTTATATCTGTGACCGTTT-CTCGAG-AAACGGTCACAGATATAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTATATCTGTGACCGTTTCTCGAGAAACGGTCACAGATATAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTATATCTGTGACCGTTTCTCGAGAAACGGTCACAGATATAAAGC-3'

Other clones with same target sequence:

  1. TRCN0000124221