Construct: shRNA TRCN0000302670

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134010.2-317s21c1
DNA Barcode:
CCGGATATTTCCTACATTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nup107 (103468)
Gene Description:
nucleoporin 107
Transcript:
RefSeq NM_134010.2 (CURRENT)
Match location:
Position 317 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302670)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGATATTTCCTACATTCTT
Hairpin Sequence:
5'-CCGG-CCGGATATTTCCTACATTCTT-CTCGAG-AAGAATGTAGGAAATATCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGATATTTCCTACATTCTTCTCGAGAAGAATGTAGGAAATATCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGATATTTCCTACATTCTTCTCGAGAAGAATGTAGGAAATATCCGG-3'

Other clones with same target sequence:

  1. TRCN0000099711