Construct: shRNA TRCN0000302709

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029091.2-1142s21c1
DNA Barcode:
CGCTATTATCAGCGGGCACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klc4 (74764)
Gene Description:
kinesin light chain 4
Transcript:
RefSeq NM_029091.2 (CURRENT)
Match location:
Position 1142 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302709)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTATTATCAGCGGGCACTA
Hairpin Sequence:
5'-CCGG-CGCTATTATCAGCGGGCACTA-CTCGAG-TAGTGCCCGCTGATAATAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTATTATCAGCGGGCACTACTCGAGTAGTGCCCGCTGATAATAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTATTATCAGCGGGCACTACTCGAGTAGTGCCCGCTGATAATAGCG-3'

Other clones with same target sequence:

  1. TRCN0000100812