Construct: shRNA TRCN0000302850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010763.2-1183s21c1
DNA Barcode:
CCTGGGATAATTACAGAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Man1a2 (17156)
Gene Description:
mannosidase, alpha, class 1A, member 2
Transcript:
RefSeq NM_010763.2 (CURRENT)
Match location:
Position 1183 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000302850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGATAATTACAGAACAT
Hairpin Sequence:
5'-CCGG-CCTGGGATAATTACAGAACAT-CTCGAG-ATGTTCTGTAATTATCCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGATAATTACAGAACATCTCGAGATGTTCTGTAATTATCCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGATAATTACAGAACATCTCGAGATGTTCTGTAATTATCCCAGG-3'

Other clones with same target sequence:

  1. TRCN0000077086