Construct: shRNA TRCN0000303153

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033523.4-1402s21c1
DNA Barcode:
AGGGATATGTTTAATCACGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spred2 (114716)
Gene Description:
sprouty-related, EVH1 domain containing 2
Transcript:
RefSeq NM_033523.4 (CURRENT)
Match location:
Position 1402 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000303153)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGATATGTTTAATCACGAA
Hairpin Sequence:
5'-CCGG-AGGGATATGTTTAATCACGAA-CTCGAG-TTCGTGATTAAACATATCCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGATATGTTTAATCACGAACTCGAGTTCGTGATTAAACATATCCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGATATGTTTAATCACGAACTCGAGTTCGTGATTAAACATATCCCT-3'

Other clones with same target sequence:

  1. TRCN0000066276