Construct: shRNA TRCN0000304370

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053268.2-1174s21c1
DNA Barcode:
TCCAGATGCCAACGCAATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rasa2 (114713)
Gene Description:
RAS p21 protein activator 2
Transcript:
RefSeq NM_053268.2 (CURRENT)
Match location:
Position 1174 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304370)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCAGATGCCAACGCAATATT
Hairpin Sequence:
5'-CCGG-TCCAGATGCCAACGCAATATT-CTCGAG-AATATTGCGTTGGCATCTGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCAGATGCCAACGCAATATTCTCGAGAATATTGCGTTGGCATCTGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCAGATGCCAACGCAATATTCTCGAGAATATTGCGTTGGCATCTGGA-3'

Other clones with same target sequence:

(none)