Construct: shRNA TRCN0000304400

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008900.2-1891s21c1
DNA Barcode:
CAACAGGGACATCTCGTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pou3f3 (18993)
Gene Description:
POU domain, class 3, transcription factor 3
Transcript:
RefSeq NM_008900.2 (CURRENT)
Match location:
Position 1891 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304400)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAGGGACATCTCGTTTAT
Hairpin Sequence:
5'-CCGG-CAACAGGGACATCTCGTTTAT-CTCGAG-ATAAACGAGATGTCCCTGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAGGGACATCTCGTTTATCTCGAGATAAACGAGATGTCCCTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAGGGACATCTCGTTTATCTCGAGATAAACGAGATGTCCCTGTTG-3'

Other clones with same target sequence:

(none)