Construct: shRNA TRCN0000304411

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011541.4-859s21c1
DNA Barcode:
GAATATTCCTCCTGATCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tcea1 (21399)
Gene Description:
transcription elongation factor A (SII) 1
Transcript:
RefSeq NM_011541.4 (CURRENT)
Match location:
Position 859 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304411)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATATTCCTCCTGATCTATT
Hairpin Sequence:
5'-CCGG-GAATATTCCTCCTGATCTATT-CTCGAG-AATAGATCAGGAGGAATATTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATATTCCTCCTGATCTATTCTCGAGAATAGATCAGGAGGAATATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATATTCCTCCTGATCTATTCTCGAGAATAGATCAGGAGGAATATTC-3'

Other clones with same target sequence:

(none)