Construct: shRNA TRCN0000304443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010461.2-2224s21c1
DNA Barcode:
TGCCTATTCACGCCGTAATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hoxb8 (15416)
Gene Description:
homeobox B8
Transcript:
RefSeq NM_010461.2 (CURRENT)
Match location:
Position 2224 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCTATTCACGCCGTAATTC
Hairpin Sequence:
5'-CCGG-TGCCTATTCACGCCGTAATTC-CTCGAG-GAATTACGGCGTGAATAGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCTATTCACGCCGTAATTCCTCGAGGAATTACGGCGTGAATAGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCTATTCACGCCGTAATTCCTCGAGGAATTACGGCGTGAATAGGCA-3'

Other clones with same target sequence:

(none)