Construct: shRNA TRCN0000304523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145125.3-4930s21c1
DNA Barcode:
ACTCGGAAGAGAGTCTATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Brwd1 (93871)
Gene Description:
bromodomain and WD repeat domain containing 1
Transcript:
RefSeq NM_145125.3 (CURRENT)
Match location:
Position 4930 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCGGAAGAGAGTCTATTTA
Hairpin Sequence:
5'-CCGG-ACTCGGAAGAGAGTCTATTTA-CTCGAG-TAAATAGACTCTCTTCCGAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCGGAAGAGAGTCTATTTACTCGAGTAAATAGACTCTCTTCCGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCGGAAGAGAGTCTATTTACTCGAGTAAATAGACTCTCTTCCGAGT-3'

Other clones with same target sequence:

(none)