Construct: shRNA TRCN0000304553

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009336.2-1249s21c1
DNA Barcode:
CAGTTCCTCCCTAGTTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps72 (21427)
Gene Description:
vacuolar protein sorting 72
Transcript:
RefSeq NM_009336.2 (CURRENT)
Match location:
Position 1249 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304553)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTTCCTCCCTAGTTCATTT
Hairpin Sequence:
5'-CCGG-CAGTTCCTCCCTAGTTCATTT-CTCGAG-AAATGAACTAGGGAGGAACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTTCCTCCCTAGTTCATTTCTCGAGAAATGAACTAGGGAGGAACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTTCCTCCCTAGTTCATTTCTCGAGAAATGAACTAGGGAGGAACTG-3'

Other clones with same target sequence:

(none)