Construct: shRNA TRCN0000304554

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009336.2-671s21c1
DNA Barcode:
GAAGTGCCCTGGGCCAATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps72 (21427)
Gene Description:
vacuolar protein sorting 72
Transcript:
RefSeq NM_009336.2 (CURRENT)
Match location:
Position 671 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304554)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGTGCCCTGGGCCAATAAT
Hairpin Sequence:
5'-CCGG-GAAGTGCCCTGGGCCAATAAT-CTCGAG-ATTATTGGCCCAGGGCACTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGTGCCCTGGGCCAATAATCTCGAGATTATTGGCCCAGGGCACTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGTGCCCTGGGCCAATAATCTCGAGATTATTGGCCCAGGGCACTTC-3'

Other clones with same target sequence:

(none)