Construct: shRNA TRCN0000304804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026768.3-660s21c1
DNA Barcode:
CCTCCCACCAGAACCATTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrps18a (68565)
Gene Description:
mitochondrial ribosomal protein S18A
Transcript:
RefSeq NM_026768.3 (CURRENT)
Match location:
Position 660 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000304804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCACCAGAACCATTATC
Hairpin Sequence:
5'-CCGG-CCTCCCACCAGAACCATTATC-CTCGAG-GATAATGGTTCTGGTGGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCACCAGAACCATTATCCTCGAGGATAATGGTTCTGGTGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCACCAGAACCATTATCCTCGAGGATAATGGTTCTGGTGGGAGG-3'

Other clones with same target sequence:

(none)