Construct: shRNA TRCN0000305017

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025872.4-443s21c1
DNA Barcode:
TCCTCGGATCCCTCCTAAATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Golt1b (66964)
Gene Description:
golgi transport 1B
Transcript:
RefSeq NM_025872.4 (CURRENT)
Match location:
Position 443 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305017)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTCGGATCCCTCCTAAATC
Hairpin Sequence:
5'-CCGG-TCCTCGGATCCCTCCTAAATC-CTCGAG-GATTTAGGAGGGATCCGAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTCGGATCCCTCCTAAATCCTCGAGGATTTAGGAGGGATCCGAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTCGGATCCCTCCTAAATCCTCGAGGATTTAGGAGGGATCCGAGGA-3'

Other clones with same target sequence:

(none)