Transcript: Mouse NM_025872.4

Mus musculus golgi transport 1B (Golt1b), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Golt1b (66964)
Length:
2796
CDS:
103..519

Additional Resources:

NCBI RefSeq record:
NM_025872.4
NBCI Gene record:
Golt1b (66964)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_025872.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000305016 TTCACGAACTAGGGTGTAAAG pLKO_005 595 3UTR 100% 10.800 15.120 N Golt1b n/a
2 TRCN0000126158 CCTGGAATCAGATCATTCGTA pLKO.1 466 CDS 100% 3.000 4.200 N Golt1b n/a
3 TRCN0000308352 CCTGGAATCAGATCATTCGTA pLKO_005 466 CDS 100% 3.000 4.200 N Golt1b n/a
4 TRCN0000133954 CCTGTTCTTTGGAATGATTCT pLKO.1 165 CDS 100% 4.950 3.960 N GOLT1B n/a
5 TRCN0000343520 CCTGTTCTTTGGAATGATTCT pLKO_005 165 CDS 100% 4.950 3.960 N GOLT1B n/a
6 TRCN0000126155 GCCTCTGATAGGCATGATCTT pLKO.1 348 CDS 100% 4.950 3.960 N Golt1b n/a
7 TRCN0000126156 GTTGGCTTTATCAGAAGAGTA pLKO.1 418 CDS 100% 4.950 3.960 N Golt1b n/a
8 TRCN0000311218 GAAAGCAACAATATGGTATAA pLKO_005 499 CDS 100% 13.200 9.240 N Golt1b n/a
9 TRCN0000305017 TCCTCGGATCCCTCCTAAATC pLKO_005 443 CDS 100% 13.200 9.240 N Golt1b n/a
10 TRCN0000134476 GATAGGCATGATCTTCGAAAT pLKO.1 354 CDS 100% 10.800 7.560 N GOLT1B n/a
11 TRCN0000343521 GATAGGCATGATCTTCGAAAT pLKO_005 354 CDS 100% 10.800 7.560 N GOLT1B n/a
12 TRCN0000126157 CGAGAGAACGTTCAGATTCTT pLKO.1 258 CDS 100% 5.625 3.938 N Golt1b n/a
13 TRCN0000308351 CGAGAGAACGTTCAGATTCTT pLKO_005 258 CDS 100% 5.625 3.938 N Golt1b n/a
14 TRCN0000126154 GCTGATTACTTCATCTTGATT pLKO.1 1484 3UTR 100% 5.625 3.938 N Golt1b n/a
15 TRCN0000159888 GTTCTTTGGAATGATTCTCTT pLKO.1 168 CDS 100% 4.950 3.465 N GOLT1B n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025872.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08197 pDONR223 100% 89.1% 97.8% None (many diffs) n/a
2 ccsbBroad304_08197 pLX_304 0% 89.1% 97.8% V5 (many diffs) n/a
3 TRCN0000471758 CCATCACGCGACAACTATGAAAAT pLX_317 100% 89.1% 97.8% V5 (many diffs) n/a
Download CSV