Construct: shRNA TRCN0000305169

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145824.4-2439s21c1
DNA Barcode:
CAGGTTGATGATAGCTTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ranbp10 (74334)
Gene Description:
RAN binding protein 10
Transcript:
RefSeq NM_145824.4 (CURRENT)
Match location:
Position 2439 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305169)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGTTGATGATAGCTTATTA
Hairpin Sequence:
5'-CCGG-CAGGTTGATGATAGCTTATTA-CTCGAG-TAATAAGCTATCATCAACCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGTTGATGATAGCTTATTACTCGAGTAATAAGCTATCATCAACCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGTTGATGATAGCTTATTACTCGAGTAATAAGCTATCATCAACCTG-3'

Other clones with same target sequence:

(none)