Construct: shRNA TRCN0000305350

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016892.3-316s21c1
DNA Barcode:
GGTATGGGCAGTAGCCAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccs (12460)
Gene Description:
copper chaperone for superoxide dismutase
Transcript:
RefSeq NM_016892.3 (CURRENT)
Match location:
Position 316 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305350)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTATGGGCAGTAGCCAATTA
Hairpin Sequence:
5'-CCGG-GGTATGGGCAGTAGCCAATTA-CTCGAG-TAATTGGCTACTGCCCATACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTATGGGCAGTAGCCAATTACTCGAGTAATTGGCTACTGCCCATACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTATGGGCAGTAGCCAATTACTCGAGTAATTGGCTACTGCCCATACC-3'

Other clones with same target sequence:

(none)