Construct: shRNA TRCN0000305379

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008509.2-1892s21c1
DNA Barcode:
TCCCTTAAGAAGGAATCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lpl (16956)
Gene Description:
lipoprotein lipase
Transcript:
RefSeq NM_008509.2 (CURRENT)
Match location:
Position 1892 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000305379)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCTTAAGAAGGAATCATTT
Hairpin Sequence:
5'-CCGG-TCCCTTAAGAAGGAATCATTT-CTCGAG-AAATGATTCCTTCTTAAGGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCTTAAGAAGGAATCATTTCTCGAGAAATGATTCCTTCTTAAGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCTTAAGAAGGAATCATTTCTCGAGAAATGATTCCTTCTTAAGGGA-3'

Other clones with same target sequence:

(none)